FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_cDNA_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_cDNA_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76202122
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC206800.017.8577231
AGCTTGA238700.017.8561331
TATGCCC249400.017.5448861
TGCCAAA300850.017.5051
ACTGAAC183950.017.4722731
CTGATAG303100.017.472081
CAATGGA250700.017.401468
GAAATGA286200.017.1752851
AAATGCC281950.017.1510581
CTACCCA313400.017.1358071
GGGCATC256200.017.0751151
TGACGGC176550.017.0445161
GGGAATG207050.017.0295771
TGATTTC261050.017.0274541
TTTATGC255350.017.004441
AGACGTT261500.016.9901181
TTGAACG209600.016.9125941
TTGGAAC227800.016.8938851
GATGCTA171800.016.8799441
TGCTACC218500.016.8678471
Produced by FastQC (version 0.11.5)