FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24219733
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGAA297600.020.0744385
CTGAAAC317350.019.918111
CCGGTAG238400.019.8130381
ACCGAGA304900.019.6351344
TACCGAG309050.019.521073
CATATTC192800.019.4107931
TGGCGAA124350.019.3948129
CTTACCG343800.019.3939151
GAAGGTT336350.019.366489
ACCTATA120000.019.2335662
AGTGAAC135250.019.213548
GTCGGAG130000.019.2057677
ACAGCCG150550.019.179111
GAGAAGG343450.019.0850357
CGACACC116450.019.042265
GTGGCGA126850.019.0290878
GCGACAC125300.019.0212424
TCATACG300950.019.0104278
CCGTGGA91850.018.9829351
ATTGGTG169800.018.9347721
Produced by FastQC (version 0.11.5)