FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_GO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_GO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43654398
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTGA172300.018.9526841
AGACTAG183900.018.7839458
GCATGCG303100.018.3837781
TTGAACG131450.018.162441
ATGCGAG142150.018.1551233
TGCCAAA155450.018.1083721
TTTATGC180850.018.0915131
TCACGTC169950.018.0704257
CCTCTGA149150.018.0070571
CATGCGA143700.017.931612
GACTAAC110600.017.8825931
ACTGATG103850.017.851451
ATTACAG164950.017.8352813
CTACCCA189700.017.834411
ACTGAGT182800.017.8008421
TCTGGAA128500.017.7701761
AAATGCC150250.017.6794871
GAATGAA123700.017.6785371
GGGAATG122600.017.6743761
GAACCTA179300.017.6386681
Produced by FastQC (version 0.11.5)