FastQCFastQC Report
Thu 6 Sep 2018
HGWHTBGX7_n01_ADT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGWHTBGX7_n01_ADT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40053088
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGAA540750.020.1406235
CGTGTCC559800.020.04073710
ACCGAGA545300.020.0206554
CTGAAAC483400.019.9467371
TACCGAG550350.019.909543
GGCGACT333300.019.8536831
ACTGAAC288300.019.8303131
CCGGTAG303300.019.7499891
GCGTGTC569700.019.7440599
CTTACCG587650.019.7398341
GAGTTGG263500.019.7081139
GAAGGTT619750.019.5912939
AGTTGGC264850.019.56010610
ATGTAAG489400.019.4842473
TGTAAGC451350.019.463474
AAGAAGC308150.019.4590710
GAGCCTC185100.019.4503383
AGAAGAA309100.019.4501178
GACTAAC203450.019.4066451
CAATAAG316600.019.3840779
Produced by FastQC (version 0.11.5)