FastQCFastQC Report
Wed 15 Aug 2018
HGN57BGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN57BGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17211538
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2714241.576988645639919No Hit
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1606580.9334319803378408Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1588740.9230668403950884No Hit
GGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC1197100.6955218063603613Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG713800.4147217988305286Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG626370.3639244790326117Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG519000.30154190752738075No Hit
GGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT413930.2404956489071459Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380630.22114816235481108No Hit
CGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA344970.20042950258134978Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG327200.1901050330307495No Hit
CGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT310730.18053587076297306Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA308280.17911240703765113Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA294470.17108871967165282Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA279640.16247240659143886Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA268610.1560639147994793Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGGAGAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC222740.12941318782783967Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCA211100.12265028261855507Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA202310.11754324337546126No Hit
CCGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC194870.11322056169529998Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGGGGAGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACT193750.1125698354208671Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA187970.10921162304031166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG137950.065.22986670
CGTGGAA72200.065.106611
ATGCCGG184650.064.7871570
CCGGGAA371350.064.762381
CCTGGAA39200.061.609731
GTGGAAA337200.061.4159772
CCGGGGA348200.061.1168671
GGTGGAA170600.060.831981
TGGGAGA177150.060.67433
CGTGAAA118400.059.4788481
CCGGAAA412650.058.925291
GTGGGAG138600.058.5352672
AGTGGAA72050.058.3438841
CGGGGGA1663200.058.264231
TGCCGTA103950.058.04687570
GCCGGGA64450.057.5664251
CGGGGAA1468000.056.1430241
TGGAAAA637950.056.004143
GCGGGGA212200.055.9165841
TGCCGCG21700.054.1933170
Produced by FastQC (version 0.11.5)