FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379199396
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG63385261.6715548776876217No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24958900.6581998880610032No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG11628930.3066705834098955No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT5998330.15818405997672No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGTCGAGTGCCTCACAGT5445690.1436101971006304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT25125800.078.510041
AGCAGTG25345650.077.635292
CAGTGGT28733150.068.3713464
AGTGGTA29148450.067.479245
GCAGTGG29312550.067.110013
GTGGTAT30612350.064.2557456
GGTATCA30705900.064.029668
TGGTATC31907750.061.56317
GTATCAA42806300.046.012849
CATGGGG19865600.035.45648224-25
AGAGTAC30362100.035.43340718-19
CAACGCA30951450.034.80557612-13
ACGCAGA31052200.034.7307214-15
AGTACAT31307000.034.29706620-21
GCAGAGT31739750.033.9717616-17
ATCAACG32192300.033.5346610-11
AAGGGTT11373250.032.68712656-57
CCTCACT11229150.032.61892372-73
CGTTCCT11223350.032.59987668-69
CAGAGTA30343900.032.47940418-19
Produced by FastQC (version 0.11.5)