FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n02_Retina_93_sample_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n02_Retina_93_sample_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28065722
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG4456611.5879192418424153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1719250.6125800006142724No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG833600.2970171228803592No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT418820.149228300629501No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGTCGAGTGCCTCACAGT365340.13017302743895204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1749650.078.475211
AGCAGTG1791550.076.454792
CAGTGGT2002150.068.275234
GCAGTGG2030200.067.402563
AGTGGTA2031550.067.333415
GGTATCA2149100.063.6508948
GTGGTAT2148500.063.632916
TGGTATC2186750.062.4964647
GTATCAA2961100.046.2818579
AGAGTAC2123700.035.07149518-19
CATGGGG1451150.035.06483524-25
CAACGCA2162450.034.54693212-13
ACGCAGA2169450.034.48105614-15
AGTACAT2223800.033.4451820-21
CCTCACT779550.032.8719472-73
TACATGG2255900.032.80731622-23
GCAGAGT2294100.032.59243816-17
ATCAACG2307700.032.4811710-11
AAGGGTT801900.032.4150656-57
CGTTCCT790800.032.40207768-69
Produced by FastQC (version 0.11.5)