FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n02_Retina_93_sample_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n02_Retina_93_sample_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16133178
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG2267061.4052160088979369No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG862430.5345691964720156No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG413940.2565768505126516No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT217040.1345302208901433No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGTCGAGTGCCTCACAGT189360.11737303090562815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT921150.075.516341
AGCAGTG928200.074.684992
CAGTGGT1025500.067.5710754
AGTGGTA1036800.066.8383565
GCAGTGG1039000.066.655443
GGTATCA1102850.062.8892948
GTGGTAT1106100.062.6905366
TGGTATC1133800.061.0569737
GTATCAA1529050.045.4268889
CATGGGG721200.034.83535424-25
AGAGTAC1094150.034.7576118-19
CAACGCA1114500.034.21575512-13
ACGCAGA1115600.034.21568314-15
AGTACAT1136150.033.40897420-21
GCAGAGT1181100.032.3195616-17
CCTCACT404000.032.31671572-73
AAGGGTT411350.032.21425256-57
TGCCTCA409350.032.2104840-41
ATCAACG1188000.032.19737610-11
CGTTCCT405800.032.14805268-69
Produced by FastQC (version 0.11.5)