FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n02_Retina_93_sample_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n02_Retina_93_sample_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22764744
Sequences flagged as poor quality0
Sequence length99
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG2049570.9003263994534707No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1537880.6755533908046583No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG395270.17363252580393612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1228900.075.975171
AGCAGTG1227900.075.801392
CAGTGGT1363500.068.175524
GCAGTGG1375950.067.5592043
AGTGGTA1387100.067.06655
GTGGTAT1454500.063.966936
GGTATCA1459500.063.7094468
TGGTATC1493900.062.20037
GTATCAA1980500.047.178019
CATGGGG811800.035.14527524-25
AGAGTAC1462200.034.7229518-19
CAACGCA1479550.034.4147212-13
GCAGAGT1484050.034.25921216-17
ACGCAGA1492600.034.17420614-15
AGTACAT1510250.033.51285620-21
ATCAACG1530700.033.3355610-11
TACATGG1535950.032.85803622-23
CAGAGTA1453850.032.05322618-19
CGCAGAG1464600.031.86763216-17
GTACATG1463500.031.61861822-23
Produced by FastQC (version 0.11.5)