FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n02_Retina_83_sample_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n02_Retina_83_sample_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15499991
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG4168712.689491884221094No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG983590.634574562011036No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG764780.4934067381071382No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT388560.25068401652620315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGTCGAGTGCCTCACAGT357090.23038077893077485No Hit
AGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTGTA164740.10628393268099316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1298200.082.2409741
AGCAGTG1338100.079.5364462
CAGTGGT1544000.068.849554
AGTGGTA1573300.067.6804055
GCAGTGG1579700.067.328613
GTGGTAT1651000.064.51446
GGTATCA1661550.064.036238
TGGTATC1723300.061.6841967
GTATCAA2429900.043.8913359
CATGGGG1120000.035.2868324-25
AGAGTAC1654050.035.2402218-19
ACGCAGA1665150.035.11523414-15
CAACGCA1672300.034.9183912-13
AGTACAT1689400.034.43528420-21
GCAGAGT1708750.034.1995916-17
ATCAACG1725600.033.89788410-11
AGTGTAT719750.033.39117448-49
AAGGGTT720000.033.36392256-57
TGCCTCA720100.033.22176440-41
CCTCACT717500.033.14742372-73
Produced by FastQC (version 0.11.5)