FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n02_Retina_83_sample_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n02_Retina_83_sample_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21294564
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG4807022.257393013540921No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1331650.6253473891271031No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG824470.38717392851997345No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT428130.20105131055982176No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGTCGAGTGCCTCACAGT422630.19846849177095152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1885900.081.9559561
AGCAGTG1914750.080.587522
CAGTGGT2201900.069.9933554
AGTGGTA2232400.069.134545
GCAGTGG2240250.068.8843843
GTGGTAT2335900.066.0645756
GGTATCA2336500.065.95678
TGGTATC2421550.063.6411937
GTATCAA3217450.047.9095239
CATGGGG1514700.036.22707724-25
AGAGTAC2314700.036.21036518-19
ACGCAGA2335650.035.96016314-15
CAACGCA2348450.035.69709412-13
AGTACAT2370400.035.3215120-21
GCAGAGT2385200.035.19821516-17
ATCAACG2428100.034.60448510-11
CCTCACT808450.034.2946972-73
AAGGGTT820500.034.1732956-57
CGTTCCT812800.033.9768468-69
TCACTAA822000.033.97311474-75
Produced by FastQC (version 0.11.5)