FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n02_Retina_83_sample_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n02_Retina_83_sample_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25680097
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG3751211.460746040016905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1808300.7041640068571392No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGTG714180.2781064261556333No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAGT391260.15235923758387673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGTCGAGTGCCTCACAGT336010.1308445213427348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1584550.076.410032
AAGCAGT1602450.075.721481
CAGTGGT1782100.067.9097064
AGTGGTA1799650.067.326195
GCAGTGG1834150.066.140593
GTGGTAT1878250.064.586396
GGTATCA1902750.063.7060558
TGGTATC2035450.059.512667
GTATCAA2629300.046.0890169
AGAGTAC1865900.035.68481418-19
CATGGGG1236550.035.2932924-25
ACGCAGA1901600.035.0649814-15
GCAGAGT1922500.034.74468216-17
CAACGCA1919350.034.72394612-13
AGTACAT1916650.034.58664720-21
ATCAACG1979950.033.70503210-11
CGCAGAG1877700.032.3938316-17
CAGAGTA1876700.032.32399418-19
AACGCAG1891850.032.05763214-15
GTACATG1890950.031.9001722-23
Produced by FastQC (version 0.11.5)