FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379199396
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC1375900.018.4758661
TGAGCCG1455900.018.245581
GGGAATG1200000.018.1923271
GATGAAA1349950.018.150211
GGGCATC1428800.017.8447441
TGACGGC1011750.017.8087161
TGTACGG1130350.017.73684710
TGCTACC1530850.017.7365171
CAATGGA1012550.017.688028
TGCACCT1905750.017.6557271
TGAGCAT1613800.017.6084941
CGCTGGA968100.017.5234491
GACTAAC911900.017.4999471
GGCTCGA1021500.017.4855251
TGGCTGG1251800.017.242341
CCGGGAT931050.017.198671
GGGACCT812850.017.1849781
CGGACTG866250.017.1562731
CTGCGGA783200.017.1368371
ACTGAAC987900.017.12121
Produced by FastQC (version 0.11.5)