FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n01_Retina_93_sample_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n01_Retina_93_sample_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28065722
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGA121200.019.1413541
CCGGGAT111150.018.4532261
AGAGCGA129500.018.3930111
GTTGGAC93650.018.3867848
GGGACCA69650.018.3311883
GAATGAA94000.018.2894381
GGAAGAC54250.018.212215
TACGGGC103900.018.1337011
GATGAAA79750.018.1335491
TGAGCAT101150.018.1283341
CAATGGA82900.018.0860338
TGGACCC94700.018.0721410
CGCTATC131300.017.9968851
ATAGCGG88500.017.9738610
TGCGTGG80900.017.9406851
GCCGGAG53600.017.923983
CGGGCCA69700.017.9113063
CGGGACC71750.017.8971022
TGACGGC58850.017.8813691
GGACCAG65950.017.8628854
Produced by FastQC (version 0.11.5)