FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n01_Retina_93_sample_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n01_Retina_93_sample_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16133178
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCG108750.019.5364821
GAGCCGA87000.019.165352
AAGAAGC93900.018.98640610
TAAGAAG84600.018.8146826
ACGATCG82850.018.654724
TGACGGC40850.018.5620061
AGAAGAA96700.018.4473928
ACACTAG79450.018.36927
GTTAACG89550.018.3609669
TCGTAGG101850.018.3298
TTAACGA89950.018.3143910
AGCCGAG122050.018.2982623
ATAGGAG63200.018.22469510
TGGACGC55300.018.193661
CAGATAG61600.018.1866667
CAACTGC87450.018.1654918
GGCGACT74800.018.12611
GGTCCTC73550.018.0868647
CACACTA79750.018.04996
CGATCGT85650.018.0447065
Produced by FastQC (version 0.11.5)