FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n01_Retina_93_sample_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n01_Retina_93_sample_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22764744
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAATG184300.020.1392781
TGCTACC311350.020.1014421
TGCACCT415750.019.959631
TGTACGG220450.019.95126210
AGCTGAG206350.019.7982510
GGGCATC157650.019.7929881
ACTGAAC123800.019.7923221
GTGTACG222350.019.7760099
TAGTGTA222000.019.7362467
GCTGTAG234950.019.613863
TGGCTGG172800.019.6014731
ATACATC207200.019.570213
CACCTCA293600.019.5580183
AACACAC284450.019.53736710
TAATAGC189950.019.5010689
CATATTC277250.019.4864231
AGTGTAC242950.019.4345788
GTAAGCA184800.019.3797848
GCTACCT284300.019.3669682
CGTAAGC178500.019.3638067
Produced by FastQC (version 0.11.5)