FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n01_Retina_83_sample_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n01_Retina_83_sample_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15499991
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGAG66150.019.0155542
GGGCATC66200.018.4508291
TTGAACG48050.018.4040241
ACGGAGA64700.018.3916111
ACACTGA89100.018.3764881
GTGAACG63500.018.3204219
TGACGGC42550.018.265281
GAAGATG38800.018.1306116
TGAGTCG62800.018.0899945
CTTAGGC56450.018.0789475
AGTGAAC76250.017.95598
CAATGGA43100.017.9052128
ACTGAAC36150.017.8964371
GAGTGAA67950.017.893227
GGAGGTA95300.017.88119110
GTAAACA63550.017.8599228
ATTGGTG100600.017.8417821
GTCGAGA70600.017.7867228
GCCTGAG39250.017.76230210
GGGACCT35550.017.7257961
Produced by FastQC (version 0.11.5)