FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n01_Retina_83_sample_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n01_Retina_83_sample_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21294564
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCCA124800.018.9798745
CCTTCCC175950.018.641861
CAATGGA83450.018.5206158
TCCAAAC160700.018.4053848
GATGCTA89750.018.3960511
TCCCTCC131850.018.371194
CTCCAAA140150.018.3394417
TGACGGC50400.018.2549251
CCAAACT144650.018.1902479
GATGAAA61600.018.1071591
CAACTAG118650.018.0668221
GGGCATC98650.018.0459231
GGCTCGA66400.017.984521
GACTAAC57800.017.8439141
AGGCGAC23900.017.7922558
GCGCGAT44800.017.7469751
CTGCGGA50800.017.5943131
CGGACTG42450.017.5913181
TGCTACC67650.017.4193341
GGGAATG48450.017.3854961
Produced by FastQC (version 0.11.5)