FastQCFastQC Report
Fri 17 Aug 2018
HGN3YBGX7_n01_Retina_83_sample_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGN3YBGX7_n01_Retina_83_sample_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25680097
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC197750.019.8217811
GATGAAA211150.019.807411
GTCGGGA155250.019.6937127
AGGCGAA106800.019.63264810
GCAGGCG190500.019.5935217
AGCAAAC193900.019.5531866
TAACGCA169050.019.3783762
TGAGCCG107550.019.3557831
GGCAGGC194600.019.342576
CTAATAG176200.019.3189073
ACGCAGA169100.019.1613124
TGAGCAT166900.019.1182861
ATAGCTA175050.019.0796056
GCAAACG164500.019.077787
AACGCAG172100.019.0714443
TTGGCAA203850.019.0637591
AAAGTTG172400.019.0136725
TGACGGC77400.018.9707181
CCGTACT221750.018.879531
AACGTGG176450.018.87485310
Produced by FastQC (version 0.11.5)