FastQCFastQC Report
Fri 10 Aug 2018
HGMTYBGX7_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGMTYBGX7_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303143112
Sequences flagged as poor quality0
Sequence length27
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGG6048030.1995107182247308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACGC8577300.020.7384721
TACTAGA10512950.020.7159654
AGGCGAA8280450.020.68496110
CGTACTA10564300.020.630722
GTACTAG10589800.020.6137623
CCGTACT10758850.020.580291
TGCGGAT6542700.020.5693472
GCGTATG10584450.020.5630722
AGTCGGT10642100.020.54176510
GTATGTC10570200.020.539594
TATGTCA10561650.020.536055
CCTATTA13007400.020.534151
ATTGCTC9581400.020.5185433
CAGGACA10638600.020.51713810
GGACGCA8490350.020.5116862
TCAGGCG8363600.020.4942538
CTGATCC6738300.020.4794241
CCACTTG9634550.020.47677810
GAGTCGG10703450.020.4751919
AGCGTAT10681100.020.4566971
Produced by FastQC (version 0.11.5)