FastQCFastQC Report
Thu 16 Aug 2018
HGLFVBGX7_n02_10XGenomics_AntMB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLFVBGX7_n02_10XGenomics_AntMB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425021044
Sequences flagged as poor quality0
Sequence length99
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25400080.597619349878591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT27409200.085.255431
AGCAGTG29654950.078.663682
GCAGTGG34040850.068.4912343
CAGTGGT34598150.067.270544
AGTGGTA36271750.064.286215
TGGTATC37646500.061.7893077
GTGGTAT37822650.061.6317986
GGTATCA38491050.060.519318
GTATCAA38899150.059.8039329
CAACGCA38105300.033.77598612-13
AGAGTAC37945850.033.7474818-19
TACATGG37435850.033.7020922-23
GCAGAGT38126500.033.698416-17
ATCAACG38718800.033.26456510-11
ACGCAGA39132350.032.93910614-15
CATGGGG18093700.032.92483524-25
CATGGGA14355150.032.74154324-25
AGTACAT39101300.032.58674220-21
CAGAGTA37911250.030.68786618-19
ACATGGG36537950.030.64303224-25
Produced by FastQC (version 0.11.5)