FastQCFastQC Report
Thu 16 Aug 2018
HGLFVBGX7_n01_10XGenomics_AntMB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLFVBGX7_n01_10XGenomics_AntMB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425021044
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTCTCTCTCTCTCTCTCTCTCTC10170660.239297798157966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAC1120750.017.929191
TTGAACG1175800.017.726751
ACTGATG1124850.017.7181551
GGGAATG964950.017.542951
GGGACCT996250.017.5189911
GACCTGG1058200.017.4771271
CAATGGA1037100.017.4381078
CGTGAGC962850.017.3848341
GATGAAA1098100.017.3758751
CTGCGGA820900.017.280221
TGACTAG1090900.017.27391
GAATGAA1056050.017.1755051
ACGAGGA979000.017.1001911
GACTGCG1077550.017.0657981
ACGAGCC980600.017.0240841
GCGCGAT995100.017.0072021
ACTGAAC964900.017.0071471
GGCTCGA1081850.016.9834021
CTCGAAA1064300.016.9624211
CCACTAC1332300.016.9501341
Produced by FastQC (version 0.11.5)