FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30737986
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA2017950.6565003966102398No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG694160.2258313215446191No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA484180.15751845290059016No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA361700.11767199061122613No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA342290.1113573283558656No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG320060.10412523448998902No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA307840.10014969751108611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC113300.042.6279561
GGCCCCA144700.037.5576023
GCCCCAG181700.035.7801864
GCGATCC24850.034.7215581
GCGACCC551200.032.013681
GCGGTCC11100.031.2825891
CAGGTCA1467950.031.0320248
AGGTCAG1488450.031.0255729
ACCCAGG80250.030.9561865
CCAGGTC1355900.030.9054097
TCAGGCG1520850.030.77613812
CCCCAGG1030850.030.463035
CGCGACC69750.030.422971
CCCAGGT1213800.030.3875526
GTCAGGC1563950.029.91452611
CAGGCGG1558200.029.91052613
CGACCCC633200.029.8643322
GGTCAGG1570400.029.74052410
ACAGGTC83650.029.1897967
GCGGACC39800.029.1699311
Produced by FastQC (version 0.11.5)