FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26709722
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA2563560.9597853545611594No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA1243270.46547470617627545No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG1145960.42904227906228304No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA1137040.42570267110979293No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG950890.3560089468546322No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC929840.34812792136136794No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA916970.3433094511429209No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA582920.21824263090420784No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA516350.19331912177895375No Hit
GCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAAT513450.19223337479888408No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT494180.18501877331407643No Hit
GGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGT465960.1744533320114676No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA422630.15823077454718548No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA381020.1426521773607378No Hit
TTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATA344640.12903166869351915No Hit
CTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTT336240.12588674640642086No Hit
GTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTA314730.11783349897838698No Hit
GTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG290980.10894160560712687No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA289030.10821153436190761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC114100.046.350191
GCGATCC29400.042.976651
GGCCCCA158500.039.195343
GCGGACC40350.038.7736471
TGCGACC82600.038.390241
GGCGACC96100.037.2220421
CGCGACC62950.037.1409841
GCCCCAG201700.036.7873574
GCGTCCC15750.035.7781561
GCGACCC599550.034.6004871
GCGGTCC13800.032.717741
CCCCCAG49950.032.1622054
CGGCCCC164250.032.02722
ACCCAGG85400.031.5574765
CAGGTCA1807050.030.1415988
AGGTCAG1852500.030.12259
GTCAGGC1884550.030.11330411
GGTCAGG1889150.030.04391310
CCAGGTC1666600.029.9516057
TCAGGCG1899850.029.91121112
Produced by FastQC (version 0.11.5)