FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31848011
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA3959611.2432832932643738No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG1461430.4588763800665605No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA1274530.400191396567905No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA1263580.39675319127464503No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG1209310.37971288065681713No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC997390.31317183355657596No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA995480.3125721100761991No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA884380.27768767098202773No Hit
GGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGT717210.2251977368382597No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA611160.19189895406655066No Hit
GCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAAT588380.1847462310911661No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT563090.1768053898248151No Hit
GTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTA535100.16801677191081102No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA524160.16458170653106094No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA496130.1557805289630175No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA404770.12709427913724344No Hit
TTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATA403230.12661073245673019No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA356730.1120101346360374No Hit
CGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATG349170.10963636002260864No Hit
CGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAAA328290.1030802206140911No Hit
CTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTT325450.10218848517730039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC160850.049.7201731
GCGATCC41500.043.096331
GCGTCCC23450.041.7909851
GGCCCCA233550.040.5513953
GCGGACC55200.039.501761
GCGGTCC15550.038.4886971
GCCCCAG297250.038.1255654
GGCGACC139650.037.0425571
CGCGACC91350.036.858191
GCGACCC885100.036.4195671
TGCGACC104250.036.3260881
CGGCCCC237000.033.581442
GCGGGCC37850.033.2892571
CGTACCC23450.033.1335032
GCGAGCC72800.031.923031
ACCCAGG123000.031.4712545
CCCCCAG67100.030.6180044
AGGTCAG2776750.030.3264719
CAGGTCA2718750.030.2955518
GTCAGGC2830800.030.29136811
Produced by FastQC (version 0.11.5)