FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26403617
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA3861141.4623526769078645No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG1508340.5712626417812378No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA878850.33285212401013087No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA787980.29843638468168965No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA692120.2621307527676984No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG684960.2594190030858272No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA620430.23497916970996813No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA613150.23222197170940634No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC582050.2204432824487645No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA532220.2015708681125014No Hit
CGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAAA525630.19907499794441044No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA431530.16343594137121442No Hit
GCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAAT365250.13833332001445106No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA326720.12374062235488419No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT318690.12069937236250623No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA317060.12008203270029254No Hit
GGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGT310660.1176581223701283No Hit
CGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCG285690.1082010847225969No Hit
CGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG277150.10496667937578402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC157200.049.1158371
GCGATCC40800.043.7500531
GCGTCCC20150.043.4243741
GCGGTCC14500.042.241431
GGCCCCA229450.039.4768333
CGCGACC98700.039.1844441
TGCGACC110450.038.945271
GGCGACC131800.038.585031
GCCCCAG291300.037.8116534
GCGGACC61100.037.4059371
GCGACCC878100.036.3552441
CGTACCC21500.032.5579342
GTCGACC25800.032.2868651
CGGCCCC234950.032.072582
GCGGGCC41900.031.3246211
GCGAGCC69550.031.2006131
CCCCCAG74000.031.1217214
GCGGCCT14250.030.9474071
AGGTCAG2750400.030.404259
CAGGTCA2692650.030.3809598
Produced by FastQC (version 0.11.5)