FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28028606
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA4576291.6327212277342658No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG1727620.6163774252633185No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA1004290.35830893623464544No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA922700.329199390080263No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA899450.32090429327808884No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG833500.2973747606284808No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA697850.24897777649020436No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC697070.2486994893716798No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA638640.22785292996733408No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA575250.20523674991185792No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA475920.16979795570282732No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA401900.14338922171156138No Hit
GGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGT393780.14049218145204936No Hit
GCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAAT388410.13857628167451497No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA382790.13657118730770984No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT372820.13301410708759473No Hit
CGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAAA354920.1266277744958133No Hit
CGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG316150.1127954775917147No Hit
CGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCG312390.11145399096908351No Hit
GTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTA284440.10148203588862036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC189700.050.461021
GCGATCC45700.045.10921
GCGGTCC15400.044.545251
GCGTCCC25550.043.561441
GGCCCCA280850.040.7007453
TGCGACC124200.038.945071
GCCCCAG353400.038.2084474
GCGGACC71050.037.6845551
GGCGACC158250.037.4437451
GCGGCCT12750.037.333161
GCGACCC1037700.036.625541
CGCGACC108000.036.036871
ACCCAGG139900.034.9747545
GCGAGCC78000.034.820351
CGGCCCC274400.034.706042
CGTACCC26550.033.8788952
GTGGCCC17250.032.260721
GTCGACC32450.032.2494621
GTGACCC51100.031.6436881
CGAACCC71000.031.598052
Produced by FastQC (version 0.11.5)