FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30977263
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA5998251.9363395662166796No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG2263550.7307133622489501No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA1355570.4376016047641136No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA1081070.34898822404032276No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA1020360.3293899787079317No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA760590.24553169852352674No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA662300.21380197469350343No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA623570.20129925616733796No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG590900.19075281118283433No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC512650.1654923483717719No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA486790.15714428999101696No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA476680.1538806059140861No Hit
CGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG419220.13533151718407144No Hit
GCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACC406500.13122527965107827No Hit
GCGCCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA363270.11726988275239165No Hit
GCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAAT316800.10226855742548979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC255200.049.990711
TGCGACC201600.043.1948851
GGCCCCA354450.041.7385983
GCGGACC79600.041.507961
GCGTCCC29300.041.212031
GGCGACC213800.040.468141
GCGGTCC23650.039.218161
GCGATCC58700.038.6971631
CGCGACC166300.038.599311
GCCCCAG462100.038.3020864
GCGACCC1396350.035.0793151
GCGAGCC92250.033.8811071
CCCCCAG151700.031.8158974
CGGCCCC401650.031.3003442
GTGGCCC22400.030.9378151
CGAACCC82600.030.846932
CGTACCC33750.030.6957662
GCGGGCC63400.030.2526741
ACCCAGG185100.030.1025185
GCCCCCA146450.030.064353
Produced by FastQC (version 0.11.5)