FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32566270
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA5370881.6492155840997451No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG2009700.6171108941859169No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA1165670.3579378295395819No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA1037880.31869784289081926No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA958690.2943812724023967No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA873510.26822537551890346No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG871640.26765116176952414No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA761130.23371727864443795No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC730870.2244254561544813No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA595220.18277192936126857No Hit
CGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCG462770.14210101433169964No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA457150.1403753024218002No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA456300.14011429617208235No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA439850.13506305757460096No Hit
GTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACG439730.13502620963346432No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT392780.12060945266375303No Hit
TTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGA388580.11931977472397054No Hit
CGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG384960.11820819516634849No Hit
CGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAAA337690.10369317702027282No Hit
CGAGGCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGG328240.10079140165576224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC216250.049.445681
GCGATCC54650.042.4616281
GCGTCCC26050.039.904511
GGCCCCA315100.039.3426323
TGCGACC153250.039.145661
GCGGTCC20400.037.7455521
GGCGACC198600.037.6439551
CGCGACC130000.036.9389081
GCCCCAG403750.036.9373934
GCGGACC80450.036.3708531
GCGACCC1205600.036.1761971
CGTACCC30950.033.925212
GTGACCC61450.032.8075521
ACCCAGG172800.032.366995
GTACCCC31050.030.772613
CCCCCAG110750.030.749384
GCGAGCC94650.030.6184351
AGGTCAG3726250.030.4482779
CAGGTCA3661550.030.3865818
ACAGGTC169600.030.2743997
Produced by FastQC (version 0.11.5)