FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33550065
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA5155561.5366766055445795No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG1861190.554750042958188No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA1144340.34108428702001026No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA965310.2877222443533269No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA935850.2789413373714775No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG872250.25998459317440964No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA868790.25895329860016664No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA810580.2416031086675987No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC722760.2154273024508298No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA641890.19132302724301728No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA574930.1713647946732741No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA447320.1333291008527107No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA440930.1314244845725336No Hit
GGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGT384240.11452734890379497No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT381610.11374344580256401No Hit
CGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG356870.10636939153471088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC196250.052.719431
GGCCCCA282450.042.3169823
GCGGACC69650.041.8098031
GCGATCC45600.041.601631
TGCGACC164350.039.377051
GCCCCAG368100.038.8603744
GCGGTCC19150.038.3818931
GCGTCCC27550.037.7320821
CGCGACC145000.036.183421
GGCGACC189750.036.061261
GCGACCC1208250.034.492251
GCGAGCC72500.034.2282071
CAAGGAT1954850.034.02441470
GCGGGCC51300.032.4079971
ACCCAGG135700.032.3693055
CGGCCCC316800.032.060962
CCCCCAG112500.030.3333424
CAGGTCA3313050.029.9384448
AGGTCAG3371400.029.8949499
GCAGACC32800.029.878591
Produced by FastQC (version 0.11.5)