FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18145356
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA2127421.1724322190206684No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG794070.4376161040874591No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA501090.27615330335761945No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA457770.25227942620690386No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA423970.23365207053529288No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG423210.23323323058528034No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA402710.22193557403888906No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA371900.20495602290745907No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC351840.19390085264791718No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA297160.1637664204549087No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA288220.15883953999028733No Hit
GCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACC211540.11658079345481015No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA190440.10495247378998793No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT181530.1000421264812881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC100600.045.298051
TGCGACC79100.041.3272861
GCGGACC33700.040.1927341
GGCGACC80900.040.1481861
GGCCCCA134350.039.1024133
CGCGACC61950.036.779531
GCGTCCC13950.036.3797951
GCCCCAG175950.035.785314
GCGATCC28250.035.43351
GCGACCC511000.033.8971371
CGAACCC37050.030.512292
GCGAGCC43550.030.4591331
GCGGGCC26900.030.0556551
CAGGTCA1533350.029.1576238
CCAGGTC1421150.029.0630877
AGGTCAG1569850.029.0514839
GTCAGGC1603550.028.83375211
CCCAGGT1268400.028.7938066
GGTCAGG1611750.028.71761510
TCAGGCG1616500.028.66106212
Produced by FastQC (version 0.11.5)