FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14971453
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA415580.27758160814451344No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA226740.15144822616749357No Hit
GAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCC215660.14404747488436828No Hit
GGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGACCCTGTCGCACC207910.1388709566132292No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA190260.12708185371186084No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG189170.12635380146469416No Hit
GTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACG181580.12128415324818506No Hit
GTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAA180800.12076316173186397No Hit
GGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGACCCTGCCGCACC172940.1155131702981668No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG165700.11067730032616073No Hit
CGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTC162200.10833951788113017No Hit
CGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGG149920.10013724118828013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC27650.035.44431
GCCCCAG43650.032.3941154
GCGACCC105200.031.5077781
TGCGACC16800.030.6260911
GGCGACC22250.030.5179421
GGCCCCA38550.030.323923
GTCAGGC351750.029.10483611
CCAGGTC305900.028.982057
CAGGTCA341350.028.8329338
TCAGGCG357900.028.64373212
CCCAGGT267950.028.5410296
AGGTCAG351450.028.5027479
GGTCAGG364100.028.18528710
CCCCAGG219400.027.9172045
ACCCAGG19550.027.2124315
TACCCGC374400.027.17581623
CCCGCTG376300.026.99128225
CAGGCGG373200.026.91601613
TTACCCG378100.026.90987822
CCGCTGA381950.026.64699426
Produced by FastQC (version 0.11.5)