FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27682512
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA5175061.869432947414599No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG1988160.7182007182007182No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA1265640.4571983929782095No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA888190.32084877268363504No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA887420.3205706187357564No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA664600.2400793685197355No Hit
GCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACC512910.18528304078762795No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA502520.181529768685732No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA486440.1757210472806803No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG412330.14894963289458701No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA402690.1454672899627028No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA395470.14285914515272313No Hit
CGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG375840.13576802567628257No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC371290.1341243887115447No Hit
GCGCCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA319340.1153580282020649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC219750.051.2382931
TGCGACC168800.044.2273451
GCGGACC68300.041.9184381
GGCCCCA311000.041.0540433
GCGATCC49900.040.7519151
GGCGACC189450.039.7575951
CGCGACC136100.039.1664541
GCCCCAG402600.038.1205254
GCGTCCC28650.038.1155741
GCGGTCC22200.035.7886541
GCGACCC1194750.035.3826561
GCGAGCC78350.035.0673641
GTCGACC30950.034.378381
GCGGGCC52600.032.7379761
ACCCAGG142850.032.5617525
CGAACCC69500.032.3303872
GTGACCC59250.032.312571
GCGGCCT16800.031.458651
CGTACCC27700.031.3352092
GCCAGGT105150.031.0220496
Produced by FastQC (version 0.11.5)