FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26009764
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA3347311.2869436262474354No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG1244290.47839342179344646No Hit
GTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAA931300.3580578432007303No Hit
GCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCA927340.3565353380369003No Hit
TGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAA873900.33598920774521446No Hit
TTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAG810030.311433044913441No Hit
TGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGC691770.26596550433906285No Hit
GCGGCCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA670580.2578185638285684No Hit
CGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAAA542440.20855244976463455No Hit
GCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAAT534930.20566507254737107No Hit
GGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA527980.20299299909064922No Hit
TGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA481760.18522274942594635No Hit
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCAT413280.1588941752797142No Hit
GGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGT388320.14929777909557349No Hit
TGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTA378280.14543769024586303No Hit
CGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA371570.1428578898293733No Hit
TTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATA331240.12735217436036714No Hit
CGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCG327590.12594885520683693No Hit
GTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTA297720.11446470640794741No Hit
AGCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATA277600.10672914986848785No Hit
GAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCC263740.1014003817950828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC133200.047.0087741
TGCGACC91900.043.226811
GCGATCC30900.042.249661
GCGGTCC12700.041.339041
GCGTCCC18350.039.86421
GGCCCCA191000.038.9763453
GGCGACC113050.038.8858761
CGCGACC79450.038.766951
GCCCCAG240000.037.31884
GCGACCC727400.037.1415981
GCGGACC51450.034.9663731
CGTACCC17900.032.262432
ACCCAGG97900.032.1400455
GTGACCC41300.031.525771
CGTCCCC22300.031.0760962
CGGCCCC199200.030.853282
CCCCCAG64100.030.7410764
CGACCCC939250.030.6642272
CAGGTCA2196350.030.6345048
AGGTCAG2243250.030.5795279
Produced by FastQC (version 0.11.5)