FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21612134
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGC311550.14415513063170904No Hit
TTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAA260270.12042771898415955No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCG251570.1164022025774965No Hit
TGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAG240180.11113201500601466No Hit
TGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCA230400.10660677932128314No Hit
GGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAGGCGAAAGGCCAT217970.10085538059314272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGC27250.037.3754770
GCGGGCG46950.033.6209644
GCGGCCC10450.033.4933781
AGCGGGC23350.032.826343
CTGCGCG12800.028.16355170
TACCGCG239100.027.98784661
CGAGGTA247100.027.35084266
GGCGGGC35650.026.9984233
ACCGCGA252650.026.59764162
CCGCGAG254400.026.51097963
AGTACCG256650.026.45584759
CGCGAGG258000.026.18175564
GCGAACA263600.025.93098652
CGATAGC264000.025.8785647
CAAGCGG16800.025.6254271
CCGATAG270700.025.28976446
GATAGCG272450.025.12720748
GAGGTAA271600.025.03820467
GCGAGGT276450.024.43441265
CAAGTAC281300.024.34912157
Produced by FastQC (version 0.11.5)