FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29791162
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA991850.33293431118933864No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG320830.10769301311576904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC37950.048.1426931
GGCCCCA59950.036.955423
GCGGACC19600.035.1788641
CGGCCCC52450.034.6324582
GCGGGCG72700.034.6147964
GCCCCAG74250.034.5992664
GCGACCC281550.033.4277341
AGCGGGC36450.032.3590663
ACCGAGC27100.030.4791470
CCAGGTC663750.029.4924247
GTCAGGC732800.029.47920411
TCAGGCG736250.029.30293712
CCCAGGT596900.029.271336
CCCGCTG733500.029.11120425
AGGTCAG732750.029.0181779
CAGGTCA720750.029.0150598
CCCCAGG522450.028.9942555
CCGCTGA737050.028.97578626
TACCCGC739850.028.95215223
GGCGACC43350.028.8237691
Produced by FastQC (version 0.11.5)