FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31589718
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCG62200.033.199114
AGCGGGC29500.032.9825443
GGCGGGC41200.029.64763
CAAGCGG24700.027.4899331
TACCGCG331400.026.94117561
CGAGGTA341800.026.03939266
GTCGGGC42250.025.8457573
AGTACCG350750.025.66444459
ACCGCGA356400.025.16920562
CCGCGAG356000.025.0795163
CTGCGCG5500.024.81756270
CGCGAGG361800.024.65811564
AAGCGGG32050.024.5705262
CGATAGC368050.024.49652747
GATAGCG370500.024.42892648
CCGATAG376250.024.03718246
GCGAACA376400.023.95283352
GAGGTAA381150.023.4888267
ACCGAGC18600.023.3327570
GAGGGCG97300.023.2012654
Produced by FastQC (version 0.11.5)