FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31358325
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCG51750.032.1933634
AGCGGGC25950.030.481523
GGCGGGC36750.027.6188893
TACCGCG294850.026.34002561
AGTACCG311700.025.21929759
CGAGGTA307150.025.00023366
ACCGAGC25950.024.68150770
ACCGCGA316400.024.65658462
CCGCGAG314800.024.57066363
GCGGCCC12000.024.5003681
CGATAGC325900.024.24955447
CGCGAGG318750.024.15637864
GATAGCG327900.024.1335948
CCGATAG331250.023.87903246
GCGAACA333700.023.77694352
GTCGGGC38650.023.272833
GAGGGCG93650.023.2088434
ACCGATA349300.022.36456545
GCGAGGT342500.022.30754565
ATAGCGA356050.022.16659749
Produced by FastQC (version 0.11.5)