FastQCFastQC Report
Sat 8 Sep 2018
HGLF5BGX7_n01_Lex01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLF5BGX7_n01_Lex01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30053947
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA619000.20596296386627688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC27400.037.0438961
AGCGGGC37150.036.742423
GCGGGCG79200.036.060584
CAAGCGG27450.032.8962631
GCGACCC172500.032.2812461
GTCAGGC431400.028.33948711
CCAGGTC392400.028.2927277
TCAGGCG432350.028.01811812
GGCGGGC54700.027.9612183
CCCAGGT357500.027.9217536
CAGGTCA428650.027.639228
TACCGCG517000.027.60001261
CCCCAGG318200.027.454465
AGGTCAG438500.027.3620349
TACCCGC438550.027.3585523
CCCGCTG438850.027.3471425
GTCGGGC56250.027.2529533
GAGGGCG131700.027.239924
CGACCCC212700.027.2327652
CCGCTGA442650.027.15190726
Produced by FastQC (version 0.11.5)