FastQCFastQC Report
Tue 7 Aug 2018
HGKMWBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165976874
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAACA7205500.065.2104170
ACGATCG11961700.058.8039631
TCGATTC5999650.058.5959472
ATACACG4053350.058.550131
TACACGA4055200.058.5473482
ATCGATT6007700.058.5462951
ACACGAT4063850.058.5405853
TCGATCG1401300.058.5174061
GATCGCG1269200.058.5087241
CACGATC4064050.058.5085074
TCGATAG12046250.058.4620865
TCGCATC7442750.058.452517
ATCGCGC1271450.058.427982
ATTCCTT6006900.058.3962335
CTGTATC6238900.058.3924674
GATAACG5965000.058.384845
TAACGCA5958350.058.3802077
AACAATG12285400.058.369552
ACGATCT4078000.058.3484155
ACTGTAT6246350.058.3225943
Produced by FastQC (version 0.11.5)