FastQCFastQC Report
Tue 7 Aug 2018
HGKMWBGX7_n01_MYCScreens2_5_BC_bc24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_MYCScreens2_5_BC_bc24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24218308
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG4439950.069.959741
TAGGTCG5446500.069.7761153
TAACAAT4814600.069.7727661
AGGTCGC5445200.069.7544254
TCGATAC4474600.069.7513964
ATAGGTC5456750.069.6920852
GGTCGCA5451900.069.6599965
TACTGTA4675800.069.648782
TCGATAG4466550.069.617215
GTCGCAT5456150.069.612596
TCGCATC5458750.069.589157
CTGTATC4683300.069.56474
ACTGTAT4683550.069.556383
GTATCTC4694400.069.461336
TGTATCT4696900.069.4209445
AACAATG4852450.069.4165042
ATACTGT4695250.069.36581
TATCTCT4704750.069.35937
ATCTCTT4705500.069.309868
AATGGTC4858600.069.2913065
Produced by FastQC (version 0.11.5)