FastQCFastQC Report
Tue 7 Aug 2018
HGKMWBGX7_n01_MYCScreens2_5_BC_bc21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_MYCScreens2_5_BC_bc21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26864523
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG5171300.069.951691
TAGGTCG6650100.069.774823
TAACAAT5249400.069.745751
AGGTCGC6653450.069.735614
TCGATAC4199450.069.726044
ATAGGTC6662350.069.6914442
GGTCGCA6660300.069.65275
TACTGTA5181200.069.635342
TCGATAG5202650.069.621855
GTCGCAT6666150.069.590916
TCGCATC6667850.069.575067
CTGTATC5189950.069.548524
ACTGTAT5192600.069.498223
GTATCTC5205350.069.401216
TGTATCT5205950.069.398875
AACAATG5291300.069.37842
ATACTGT5201200.069.361131
TATCTCT5215200.069.336067
ATCTCTT5216450.069.271138
CGATAGG5226850.069.247666
Produced by FastQC (version 0.11.5)