FastQCFastQC Report
Tue 7 Aug 2018
HGKMWBGX7_n01_MYCScreens2_5_BC_bc20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_MYCScreens2_5_BC_bc20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21278906
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGTCCTCAGCATCTTAT458210.21533531846045095No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGTCCTCAGCATCTTATC428480.20136373552286946No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTCCTCAGCATCTTATCC422890.19873672076938542No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTCCTCAGCA416160.19557396418782058No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTCCTCAGCAT405490.19055960865657287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG4095850.069.954911
TAACAAT4226350.069.741111
TAGGTCG4645000.069.735693
TCGATAC3930150.069.732544
AGGTCGC4644700.069.70154
ATAGGTC4653400.069.663312
TACTGTA4052950.069.6429442
TCGATAG4118300.069.642485
GGTCGCA4648700.069.6245965
GTCGCAT4653300.069.583936
CTGTATC4055700.069.567524
TCGCATC4654350.069.565267
ACTGTAT4057150.069.547343
GTATCTC4065300.069.461546
TGTATCT4066800.069.447645
TATCTCT4072250.069.4048167
ATACTGT4069200.069.389441
AACAATG4260000.069.3729552
ATCTCTT4076500.069.296528
CGATAGG4138000.069.257966
Produced by FastQC (version 0.11.5)