FastQCFastQC Report
Tue 7 Aug 2018
HGKMWBGX7_n01_MYCScreens2_5_BC_bc17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_MYCScreens2_5_BC_bc17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20343091
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC498330.24496277384788773No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG471870.23195590090021226No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG470890.23147416486511319No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG468180.23014201725784939No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC392240.19281239021149735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG4097250.069.960831
TAACAAT4126700.069.783871
TAGGTCG4385950.069.762133
AGGTCGC4386300.069.7178044
ATAGGTC4395650.069.6829762
TACTGTA4189200.069.652962
TCGATAC3228800.069.6495064
GGTCGCA4390350.069.63925
TCGATAG4118150.069.629415
CTGTATC4191600.069.607594
GTCGCAT4393400.069.600756
ACTGTAT4192100.069.5997853
TCGCATC4395550.069.578887
TGTATCT4201400.069.4844065
GTATCTC4200450.069.455476
ATACTGT4205250.069.416761
TATCTCT4206900.069.389327
AACAATG4162250.069.363922
ATCTCTT4208250.069.337958
AATGGTC4165600.069.2614445
Produced by FastQC (version 0.11.5)