FastQCFastQC Report
Tue 7 Aug 2018
HGKMWBGX7_n01_MYCScreens2_5_BC_bc16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_MYCScreens2_5_BC_bc16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22128806
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGT386210.17452816930113627No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTT366950.1658245817691203No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC355400.16060514064789577No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA353580.15978268325909675No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTC347670.15711195624382085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG4400500.069.958661
TAACAAT4243000.069.766381
TCGATAC4102650.069.746694
TAGGTCG4839250.069.7370763
AGGTCGC4842900.069.688774
ATAGGTC4850700.069.645752
TCGATAG4425200.069.6347665
TACTGTA4204200.069.6162952
GGTCGCA4847450.069.6093755
CTGTATC4207450.069.571794
GTCGCAT4851850.069.560996
TCGCATC4853450.069.542987
ACTGTAT4209600.069.53233
GTATCTC4216550.069.4514856
TGTATCT4218500.069.4373255
TATCTCT4224300.069.3976067
ATACTGT4219000.069.387541
AACAATG4280000.069.368842
ATCTCTT4225950.069.334548
CGATAGG4446350.069.268426
Produced by FastQC (version 0.11.5)