FastQCFastQC Report
Tue 7 Aug 2018
HGKMWBGX7_n01_MYCScreens2_5_BC_bc13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_MYCScreens2_5_BC_bc13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18185570
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGT780910.4294118908563218No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTC747920.41127113420145756No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA736730.405117903920526No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTT722620.3973590049693246No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC679210.37348843066233284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG3518750.069.950831
TAACAAT3593950.069.769841
TAGGTCG3897600.069.7564543
TCGATAC3658350.069.722334
AGGTCGC3898550.069.699444
ATAGGTC3905450.069.694732
TCGATAG3536500.069.644625
TACTGTA3237350.069.615412
GGTCGCA3902400.069.6077355
ACTGTAT3240050.069.5599443
GTCGCAT3905850.069.545736
TCGCATC3906850.069.5293667
CTGTATC3242500.069.517484
AACAATG3623200.069.4309162
TGTATCT3249700.069.414475
GTATCTC3250700.069.389186
ATACTGT3252550.069.337241
TATCTCT3257650.069.313877
CGATAGG3552600.069.2633746
ATCTCTT3258800.069.253978
Produced by FastQC (version 0.11.5)