FastQCFastQC Report
Tue 7 Aug 2018
HGKMWBGX7_n01_MYCScreens2_5_BC_bc09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_MYCScreens2_5_BC_bc09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21443163
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG3157000.069.9428941
TCGATTC4753550.069.853822
ATCGATT4755200.069.819121
TCGATAA6833800.069.792133
TAACAAT3347650.069.745621
GATAACG6836500.069.664165
ATAACGC6833650.069.64546
CGATAAC6843550.069.642044
ATTCCTT4759800.069.630625
TAACGCA6832900.069.622897
TCGATAG3179700.069.556535
AACGCAT6840650.069.546898
CGATTCC4766900.069.545773
ACGCATT6843950.069.507569
TCGATAC3064350.069.455764
TTGGTTC4757400.069.4114710
GATTCCT4780450.069.386474
TTCCTTG4775400.069.366846
GGTTCTT4760600.069.307312
CGCATTT6858950.069.2857810
Produced by FastQC (version 0.11.5)