FastQCFastQC Report
Tue 7 Aug 2018
HGKMWBGX7_n01_MYCScreens2_5_BC_bc08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_MYCScreens2_5_BC_bc08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27456205
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGCTGTAGTAATTCCAGC486330.17712935928326584No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGCTGTAGTAATTCCAG278390.1013942021484761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACG6225750.069.895881
TACACGA6222400.069.886822
GATCGCG1531700.069.837011
CGATCGT3818900.069.826932
TCGATCG3821900.069.825241
ATCGCGC1531250.069.807242
TAAGTAG12105400.069.790441
ACACGAT6231900.069.7902153
CACGATC6231000.069.7728964
ATCGTTA3813600.069.760194
ACGATCT6238750.069.739915
TCGTTAC3814300.069.730345
TCGCGCG1533500.069.650413
AAGTAGA12147650.069.569892
TAGAGTC12147700.069.5148855
ATGATCG3380500.069.502796
GATCGTT3833650.069.483793
GTAGAGT12159950.069.464054
GATCGTC3381250.069.4505848
CGATCAT3371650.069.450511
Produced by FastQC (version 0.11.5)