FastQCFastQC Report
Tue 7 Aug 2018
HGKMWBGX7_n01_MYCScreens2_5_BC_bc01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGKMWBGX7_n01_MYCScreens2_5_BC_bc01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26054006
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGCTGTAGTAATTCCAG1742260.6687109844067741No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG1195500.4588545807504612No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGCTGTAGTAATTCCAGC1139650.4374183378939883No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG758290.29104545381620006No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGCTGTAGTAATTCCA665880.2555768199331803No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC452790.1737890134822261No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGCTGTAGTAATTC373640.14340980807327672No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGCTGTAGTAATTCC332430.12759266271758746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG2155500.069.979151
ATACACG10944950.069.9392241
TACACGA10942950.069.9262852
GATCGCG3906700.069.907641
ATCGCGC3909000.069.866372
ACACGAT10957300.069.853683
CACGATC10959200.069.8191154
CGATCGT2161100.069.776622
ACGATCT10973500.069.77625
TCGCGCG3914050.069.768313
ATCGTTA2157150.069.759034
TCGTTAC2157800.069.714225
TAAGTAG6784150.069.686721
CGATCTC10994950.069.617686
ATCTCTT11040300.069.560858
GCGCGGT3919300.069.559835
GATCTCT11012750.069.546647
GCGGTTC3920300.069.489287
TCTCTTG11048200.069.482379
TAGAGTC6801750.069.474535
Produced by FastQC (version 0.11.5)