FastQCFastQC Report
Wed 18 Jul 2018
HGG2LBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGG2LBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13701171
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG263600.19239231449633026No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239330.1746785001077645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG128150.062.16632515
GCACACG130950.061.24380511
ACACGTC133500.060.07682813
CACACGT134650.059.74278612
CACGTCT135550.058.66747714
CGTCTGA136750.058.00105716
AGCACAC146650.055.96740710
CTGAACT145750.055.08232519
TCGGAAG154350.054.5813983
AGAGCAC152850.053.9340868
GAACTCC151450.052.64150221
GATCGGA158750.051.8586041
ATCGGAA163800.051.6273842
CGGAAGA166750.051.001254
TGAACTC162850.049.68276220
CAGTCAC107150.049.2826127
CCAGTCA119600.048.7694826
CTCCAGT145200.048.281724
ACTCCAG171050.044.00700823
TCCAGTC150500.043.56331625
Produced by FastQC (version 0.11.5)